Hello Santanu,
Why dont u try DSP - di thiobissuccinimydal propionate. You can get a few
technical references from the PierceNet website. Its a commonly used
immobilizing agent onto gold.
Best wishes,
Samir
Samir Kagadkar
Department of Electrical Engg
IIT Bombay
> I need help to find a suitable thiol reagent to form a
> coupling between goat anti-mouse IgM (miu chain) and
> bare gold surface. It would be great if anybody can
> tell me how to select thiol reagents in this kind of
> situation. I am having problem in immobilizing this
> antibody on the gold surface .
> Santanu Chandra